Antioxidative Responses of Chlorella vulgaris Under Different Growth Phases

Chlorella vulqaris is a unicellular microorganism that offers health benefits due to its concentrated antioxidant production. This microalga has received huge attention natural antioxidative property as an alternative source because of rapid growth, easy and flexible culture. Research date only focuses on the growth production in selected phase, especially exponential stationary phases; however, so far, limited reports antioxidants all phases C. vulgaris. Thus, this study determines enzymatic (Catalase, CAT; Ascorbate Peroxidase, APX; guaiacol peroxidase, gPOD) specific activities amount non-enzymatic (a-tocopherol, ascorbic acid carotenoids) vulgaris five phases. was cultured F/2 medium at 25±2 °C under laboratory conditions. CAT were highest phase (1.50±0.08 units/mg protein), whereas APX gPOD induced lag 37.13±4.93 protein 1.31±0.03 protein, respectively. The a-tocopherol accumulated (97.3±4.18 µg/g.fwt), (266.67±22.22 µg/g.fwt) carotenoids (8.16±2.52 decline phase. Production microalgae cells indicated they efficiently scavenged reactive oxygen species (ROS) converted them into less harmful substances. In addition, these different can be used guideline produce massive antioxidants, which commercialized food pharmaceutical industries.